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rmc 4550 shp2 allosteric inhibitors (MedChemExpress)

Name: rmc 4550 shp2 allosteric inhibitors
Brand: MedChemExpress
Rating: 94 (17 reviews)

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MedChemExpress rmc 4550 shp2 allosteric inhibitors

(i) Representative blots of Shp1 and <t>Shp2</t> expression levels from CT, Shp1, Shp2 and Shp1/2 DKO mice by capillary immunoassays with the respective antibodies in (A) sorted-MK progenitors and (B) washed platelets. Percentage of residual level of (ii) Shp1 and (iii) Shp2 in Shp1 KO (blue), Shp2 KO (green) and Shp1/2 DKO (red) mice. n = 3-6 mice per genotype. Mean ± SEM; one way-ANOVA; ** P < 0.01, *** P < 0.001.

Rmc 4550 Shp2 Allosteric Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rmc 4550 shp2 allosteric inhibitors/product/MedChemExpress
Average 94 stars, based on 17 article reviews

rmc 4550 shp2 allosteric inhibitors - by Bioz Stars, 2026-02

94/100 stars

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1) Product Images from "Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice"

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

Journal: bioRxiv

doi: 10.1101/2025.10.24.684367

(i) Representative blots of Shp1 and Shp2 expression levels from CT, Shp1, Shp2 and Shp1/2 DKO mice by capillary immunoassays with the respective antibodies in (A) sorted-MK progenitors and (B) washed platelets. Percentage of residual level of (ii) Shp1 and (iii) Shp2 in Shp1 KO (blue), Shp2 KO (green) and Shp1/2 DKO (red) mice. n = 3-6 mice per genotype. Mean ± SEM; one way-ANOVA; ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: (i) Representative blots of Shp1 and Shp2 expression levels from CT, Shp1, Shp2 and Shp1/2 DKO mice by capillary immunoassays with the respective antibodies in (A) sorted-MK progenitors and (B) washed platelets. Percentage of residual level of (ii) Shp1 and (iii) Shp2 in Shp1 KO (blue), Shp2 KO (green) and Shp1/2 DKO (red) mice. n = 3-6 mice per genotype. Mean ± SEM; one way-ANOVA; ** P < 0.01, *** P < 0.001.

Techniques Used: Expressing

(A) Platelet counts (i) and platelet volumes (ii) of control (CT) (n=75), Shp1 KO (n=21), Shp2 KO (n=29) and Shp1/2 DKO (n=46) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001. (B) (i) Cumulative bleeding time was recorded (ii) and the volume of blood loss was measured over 30 minutes. Mean ± SEM; one way-ANOVA; * P < 0.05. (C) Mean fluorescence intensity (MFI) of platelet (i) GPVI and (ii) α2 integrin expression in CT (n=10), Shp1 KO (n=4), Shp2 KO (n=4) and Shp1/2 DKO (n=13) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001.

Figure Legend Snippet: (A) Platelet counts (i) and platelet volumes (ii) of control (CT) (n=75), Shp1 KO (n=21), Shp2 KO (n=29) and Shp1/2 DKO (n=46) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001. (B) (i) Cumulative bleeding time was recorded (ii) and the volume of blood loss was measured over 30 minutes. Mean ± SEM; one way-ANOVA; * P < 0.05. (C) Mean fluorescence intensity (MFI) of platelet (i) GPVI and (ii) α2 integrin expression in CT (n=10), Shp1 KO (n=4), Shp2 KO (n=4) and Shp1/2 DKO (n=13) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001.

Techniques Used: Control, Fluorescence, Expressing

(A-C) Aggregation of washed platelets were measured by lumi-aggregometry in response to agonists indicated. Representative traces, n= 4-8 mice/genotype per condition, percentage of aggregation at 5 minutes and area under the curve (AUC) quantification. Mean ± SEM, n = 5-17 mice/genotype per condition, one way-ANOVA, * P < 0.05. (D) Mean fluorescence intensity (MFI) of P-selectin expression of control (CT), Shp1 KO, Shp2 KO and Shp1/2 DKO platelets in whole blood in response to (i) 1 and 3 μg/mL CRP and (ii) 100 and 500 μM PAR4 peptide (PAR4p). Mean ± SEM, n = 5-9 mice/genotype per condition, two way-ANOVA; * P < 0.05, *** P < 0.001.

Figure Legend Snippet: (A-C) Aggregation of washed platelets were measured by lumi-aggregometry in response to agonists indicated. Representative traces, n= 4-8 mice/genotype per condition, percentage of aggregation at 5 minutes and area under the curve (AUC) quantification. Mean ± SEM, n = 5-17 mice/genotype per condition, one way-ANOVA, * P < 0.05. (D) Mean fluorescence intensity (MFI) of P-selectin expression of control (CT), Shp1 KO, Shp2 KO and Shp1/2 DKO platelets in whole blood in response to (i) 1 and 3 μg/mL CRP and (ii) 100 and 500 μM PAR4 peptide (PAR4p). Mean ± SEM, n = 5-9 mice/genotype per condition, two way-ANOVA; * P < 0.05, *** P < 0.001.

Techniques Used: Fluorescence, Expressing, Control

Whole cell lysates of resting and (A) 10 µg/ml CRP-stimulated and (B) 10 µg/ml activating CLEC-2 antibody platelets from Shp1 KO, Shp2 KO and Shp1/2 DKO mice and litter-matched CT mice were western blotted with anti-Src p-Ty418, Src total, -Syk p-Tyr519/520 and Syk antibodies. (i) Representative blots of capillary-based immunoassays and (ii) quantification of peak areas from three independent experiments, Mean ± SEM, n = 3-4 mice/genotype; one-way ANOVA, *** P < 0.001.

Figure Legend Snippet: Whole cell lysates of resting and (A) 10 µg/ml CRP-stimulated and (B) 10 µg/ml activating CLEC-2 antibody platelets from Shp1 KO, Shp2 KO and Shp1/2 DKO mice and litter-matched CT mice were western blotted with anti-Src p-Ty418, Src total, -Syk p-Tyr519/520 and Syk antibodies. (i) Representative blots of capillary-based immunoassays and (ii) quantification of peak areas from three independent experiments, Mean ± SEM, n = 3-4 mice/genotype; one-way ANOVA, *** P < 0.001.

Techniques Used: Western Blot

(A) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO, and litter-matched CT mice were stained with propidium iodide and ploidy of cells was quantified by flow cytometry. (i-ii) The percentage of 2-4N and 8-128N ploidy cells was quantified (n = 4-6 mice/genotype; mean ± SEM; two-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001). (B) Ex vivo proplatelet formation. Percentage of MKs forming proplatelet was quantified in culture. Mean ± SEM, two-way ANOVA, *** P < 0.001. (C) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice were stimulated with 50 ng/mL thrombopoietin (Tpo) for 10 min at 37°C. Whole cell lysates were western blotted with indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype; two-way ANOVA, *** P < 0.001.

Figure Legend Snippet: (A) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO, and litter-matched CT mice were stained with propidium iodide and ploidy of cells was quantified by flow cytometry. (i-ii) The percentage of 2-4N and 8-128N ploidy cells was quantified (n = 4-6 mice/genotype; mean ± SEM; two-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001). (B) Ex vivo proplatelet formation. Percentage of MKs forming proplatelet was quantified in culture. Mean ± SEM, two-way ANOVA, *** P < 0.001. (C) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice were stimulated with 50 ng/mL thrombopoietin (Tpo) for 10 min at 37°C. Whole cell lysates were western blotted with indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype; two-way ANOVA, *** P < 0.001.

Techniques Used: Derivative Assay, Staining, Flow Cytometry, Ex Vivo, Western Blot

Bone marrow explants. Proportion of MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice extending proplatelets at 6h of observation were observed. Bars represent the mean ± SEM of six independent experiments. (A) Quantification and (B) representatives’ images of round MKs, MKs with large ends and proplatelet (PPT) forming MKs. Scale bar: 50 µm. Mean ± SEM, one-way ANOVA, * P < 0.1, *** P < 0.001. (C) Classification of the MK according to their maturation stage: stage I (absence of granules), stage II (granules and developing demarcation membrane system (DMS) not yet organized), stage III (DMS organized in cytoplasmic territories). Data are reported as the percentage of the total number of MK, (i) of all stage MK and (ii) only stage 3 MK. Bars represent the mean ± SEM in three BM samples, *** P < 0.001. (D) Ploidy of in situ BM MKs measured by flow cytometry. Mean ± SEM, two-way ANOVA, * P < 0.1, *** P < 0.001.

Figure Legend Snippet: Bone marrow explants. Proportion of MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice extending proplatelets at 6h of observation were observed. Bars represent the mean ± SEM of six independent experiments. (A) Quantification and (B) representatives’ images of round MKs, MKs with large ends and proplatelet (PPT) forming MKs. Scale bar: 50 µm. Mean ± SEM, one-way ANOVA, * P < 0.1, *** P < 0.001. (C) Classification of the MK according to their maturation stage: stage I (absence of granules), stage II (granules and developing demarcation membrane system (DMS) not yet organized), stage III (DMS organized in cytoplasmic territories). Data are reported as the percentage of the total number of MK, (i) of all stage MK and (ii) only stage 3 MK. Bars represent the mean ± SEM in three BM samples, *** P < 0.001. (D) Ploidy of in situ BM MKs measured by flow cytometry. Mean ± SEM, two-way ANOVA, * P < 0.1, *** P < 0.001.

Techniques Used: Membrane, In Situ, Flow Cytometry

(A) Effects of two selective Shp1 allosteric inhibitors 10 µM M029 and 10 µM F2Ac were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, and (iii) MK ploidy. Quantification from n = 3-4 independent experiments/condition. (B) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. (C) Effects of two selective Shp2 allosteric inhibitors 10 µM SHP099 and 10 µM RMC-4550 were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, (iii) MK ploidy and (iv) percentage of MKs forming proplatelets. Quantification from n = 3-6 independent experiments/condition. * P < 0.05; ** P < 0.01; *** P < 0.001, two-way ANOVA. (D) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. * P < 0.05; ** P < 0.01; two-way ANOVA.

Figure Legend Snippet: (A) Effects of two selective Shp1 allosteric inhibitors 10 µM M029 and 10 µM F2Ac were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, and (iii) MK ploidy. Quantification from n = 3-4 independent experiments/condition. (B) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. (C) Effects of two selective Shp2 allosteric inhibitors 10 µM SHP099 and 10 µM RMC-4550 were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, (iii) MK ploidy and (iv) percentage of MKs forming proplatelets. Quantification from n = 3-6 independent experiments/condition. * P < 0.05; ** P < 0.01; *** P < 0.001, two-way ANOVA. (D) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. * P < 0.05; ** P < 0.01; two-way ANOVA.

Techniques Used: Western Blot

Image Search Results

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: (i) Representative blots of Shp1 and Shp2 expression levels from CT, Shp1, Shp2 and Shp1/2 DKO mice by capillary immunoassays with the respective antibodies in (A) sorted-MK progenitors and (B) washed platelets. Percentage of residual level of (ii) Shp1 and (iii) Shp2 in Shp1 KO (blue), Shp2 KO (green) and Shp1/2 DKO (red) mice. n = 3-6 mice per genotype. Mean ± SEM; one way-ANOVA; ** P < 0.01, *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Expressing

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: (A) Platelet counts (i) and platelet volumes (ii) of control (CT) (n=75), Shp1 KO (n=21), Shp2 KO (n=29) and Shp1/2 DKO (n=46) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001. (B) (i) Cumulative bleeding time was recorded (ii) and the volume of blood loss was measured over 30 minutes. Mean ± SEM; one way-ANOVA; * P < 0.05. (C) Mean fluorescence intensity (MFI) of platelet (i) GPVI and (ii) α2 integrin expression in CT (n=10), Shp1 KO (n=4), Shp2 KO (n=4) and Shp1/2 DKO (n=13) mice. Mean ± SEM; one way-ANOVA; *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Control, Fluorescence, Expressing

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: (A-C) Aggregation of washed platelets were measured by lumi-aggregometry in response to agonists indicated. Representative traces, n= 4-8 mice/genotype per condition, percentage of aggregation at 5 minutes and area under the curve (AUC) quantification. Mean ± SEM, n = 5-17 mice/genotype per condition, one way-ANOVA, * P < 0.05. (D) Mean fluorescence intensity (MFI) of P-selectin expression of control (CT), Shp1 KO, Shp2 KO and Shp1/2 DKO platelets in whole blood in response to (i) 1 and 3 μg/mL CRP and (ii) 100 and 500 μM PAR4 peptide (PAR4p). Mean ± SEM, n = 5-9 mice/genotype per condition, two way-ANOVA; * P < 0.05, *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Fluorescence, Expressing, Control

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: Whole cell lysates of resting and (A) 10 µg/ml CRP-stimulated and (B) 10 µg/ml activating CLEC-2 antibody platelets from Shp1 KO, Shp2 KO and Shp1/2 DKO mice and litter-matched CT mice were western blotted with anti-Src p-Ty418, Src total, -Syk p-Tyr519/520 and Syk antibodies. (i) Representative blots of capillary-based immunoassays and (ii) quantification of peak areas from three independent experiments, Mean ± SEM, n = 3-4 mice/genotype; one-way ANOVA, *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Western Blot

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: (A) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO, and litter-matched CT mice were stained with propidium iodide and ploidy of cells was quantified by flow cytometry. (i-ii) The percentage of 2-4N and 8-128N ploidy cells was quantified (n = 4-6 mice/genotype; mean ± SEM; two-way ANOVA, * P < 0.05, ** P < 0.01, *** P < 0.001). (B) Ex vivo proplatelet formation. Percentage of MKs forming proplatelet was quantified in culture. Mean ± SEM, two-way ANOVA, *** P < 0.001. (C) Mature BM-derived MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice were stimulated with 50 ng/mL thrombopoietin (Tpo) for 10 min at 37°C. Whole cell lysates were western blotted with indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype; two-way ANOVA, *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Derivative Assay, Staining, Flow Cytometry, Ex Vivo, Western Blot

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: Bone marrow explants. Proportion of MKs from Shp1 KO, Shp2 KO, Shp1/2 DKO and litter-matched CT mice extending proplatelets at 6h of observation were observed. Bars represent the mean ± SEM of six independent experiments. (A) Quantification and (B) representatives’ images of round MKs, MKs with large ends and proplatelet (PPT) forming MKs. Scale bar: 50 µm. Mean ± SEM, one-way ANOVA, * P < 0.1, *** P < 0.001. (C) Classification of the MK according to their maturation stage: stage I (absence of granules), stage II (granules and developing demarcation membrane system (DMS) not yet organized), stage III (DMS organized in cytoplasmic territories). Data are reported as the percentage of the total number of MK, (i) of all stage MK and (ii) only stage 3 MK. Bars represent the mean ± SEM in three BM samples, *** P < 0.001. (D) Ploidy of in situ BM MKs measured by flow cytometry. Mean ± SEM, two-way ANOVA, * P < 0.1, *** P < 0.001.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Membrane, In Situ, Flow Cytometry

Journal: bioRxiv

Article Title: Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice

doi: 10.1101/2025.10.24.684367

Figure Lengend Snippet: (A) Effects of two selective Shp1 allosteric inhibitors 10 µM M029 and 10 µM F2Ac were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, and (iii) MK ploidy. Quantification from n = 3-4 independent experiments/condition. (B) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. (C) Effects of two selective Shp2 allosteric inhibitors 10 µM SHP099 and 10 µM RMC-4550 were tested on megakaryopoeisis. (i) Viability, (ii) proliferation, (iii) MK ploidy and (iv) percentage of MKs forming proplatelets. Quantification from n = 3-6 independent experiments/condition. * P < 0.05; ** P < 0.01; *** P < 0.001, two-way ANOVA. (D) Whole cell lysates of resting and 50 ng/mL Tpo-stimulated MKs were western blotted with the indicated antibodies. Representative blots capillary-based immunoassays and quantification of peak areas from n = 3 independent experiments/genotype. * P < 0.05; ** P < 0.01; two-way ANOVA.

Article Snippet: SHP099 and RMC-4550 Shp2 allosteric inhibitors were purchased from MedChemExpress.

Techniques: Western Blot

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1) Product Images from "Synergistic effects of deleting the tyrosine phosphatases Shp1 and Shp2 on megakaryopoiesis and thrombopoiesis in mice"

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